human derived breast carcinoma cell lines mcf 7  (ATCC)

Journal: Journal of lipid research

Article Title: Identification of Egr1 as the oncostatin M-induced transcription activator that binds to sterol-independent regulatory element of human LDL receptor promoter.

doi: 10.1194/jlr.m200126-jlr200

Figure Lengend Snippet: Fig. 9. A SIRE mutation abolishes OM induction of LDLR pro- moter activity in HepG2 and T47D-TAM67 cells. LDLR promoter wild-type vector pLDLR234Luc and the SIRE mutant vector pLDLR-SIREmu6 were separately transfected into HepG2 and T47D-TAM67 cells. Twenty hours after transfection, cells were treated with OM (50 ng/ml) for 4 h prior to cell lysis. The normal- ized luciferase activity is expressed as the fold of luciferase activity in untreated control cells.

Article Snippet: The human hepatoma cell line HepG2 and the human breast carcinoma-derived cell line T47D were obtained from American Type Culture Collection (Manassas, VA).

Techniques: Mutagenesis, Activity Assay, Plasmid Preparation, Transfection, Lysis, Luciferase, Control

Journal: Journal of lipid research

Article Title: Identification of Egr1 as the oncostatin M-induced transcription activator that binds to sterol-independent regulatory element of human LDL receptor promoter.

doi: 10.1194/jlr.m200126-jlr200

Figure Lengend Snippet: Fig. 10. Activation of LDLR promoter activity by coexpression of Egr1 with c/EBP or with CREB in T47D-TAM67 cells. The LDLR promoter reporter pLDLR234Luc was cotransfected with pCMV- Egr1 or with pCMV5 in the absence (mock) or the presence of equal amounts of ATF-2, ATF-3, c/EBP, or CREB expression vec- tors into T47D-TAM67 cells. Forty hours after transfection, cells were treated either with OM (50 ng/ml) or with OM dilution buffer for 4 h prior to harvesting cell lysates. The normalized lu- ciferase activity is expressed as the fold of luciferase activity in un- treated control cells (mock).

Article Snippet: The human hepatoma cell line HepG2 and the human breast carcinoma-derived cell line T47D were obtained from American Type Culture Collection (Manassas, VA).

Techniques: Activation Assay, Activity Assay, Expressing, Transfection, Luciferase, Control